p.p1 Rice produce pro-vitamin A. The second step

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  Genetic engineering is a widely used biotechnological method to directly manipulate an organism’s genome, and the Genetically modified organisms (GMOs) are the animal or plant that has been created through genetic engineering to increase the crop yield or to improve the nutrient value in animal. But how do we make a GMO, or in the other hand, what is the process of genetic engineering to make GMOs? All the products of genetic engineering are basically using the same steps. The first step is in order to identify a desirable new trait, and it is also the hardest procedure because the discovery of the new trait often has a big uncertainty and may need thousands of experiments and trials to test. For example, a famous biotechnology company, Syngenta, wanted to search for a brand new kind of Golden Rice with an increased amount of pro-vitamin A. The researchers at Syngenta first need to identify the gene sequence that produce this special kind of vitamin, and then screened a list of plants that they hypothesize will produce the nutrient of interest with that sequence. With the combination of critical thinking and luck, they eventually found out that maize contained a gene that would make Golden Rice produce pro-vitamin A. The second step is to Isolate the genetic trait of interest. We use a method called Comparative analysis to compare the genomes of plants with the trait to genomes in the same species without the trait, and that helps to decode what part of an organism’s genetic makeup contains the trait of interest. Monsanto company also developed a method known as seed chipping to accelerate this process. This creates a genetic database for plants, where a barcode system is used to match plants to their genotypes. Researchers then use this database to identify new traits of interest as well as to optimize the desirable traits in the organisms by selecting for the best genotypes based on plant phenotypes. Step three is to insert the desired genetic trait into a new genome. Inserting the desired genetic trait into a new genome is very difficult because it’s hard to change or rearrange the genomes due to their rigid structure. Thus, many biotech companies use “gene guns” that shoot metal particles coated with DNA into plant tissue. Monsanto invents another method that takes advantage of bacteria, called Agrobacterium tumefacient. This new method naturally invades seeds and alter plants by inserting pieces of their own DNA into a plant’s genome. Genetic trait is cut and pasted into a plasmid using enzymes, then insert the plasmid into bacteria that finally infects cells. After a genetic trait has been successfully inserted into an organism’s genome, the modified organism must then be able to grow and replicate with its newly engineered genome.